R&D Learning Pathway

Bioprocessing Scale-up

Pre-training
Assessment, orientation,
access to preparatory material
and support (virtual)
In Lab Training
Hands-on laboratory work,
practical skills,
interactive case studies
(in person)
Post-training
Assessment and
Action Plan
(virtual)
Networking
Making new connections.
Follow-up
Program Impact
Survey
(virtual)
R&D Learning Pathway

Bioprocessing Scale-up

This two-day hands-on training introduces trainees to bioprocessing from upstream to downstream. Working in the BioZone labs, participants gain practical experience with laboratory and pilot scale bioreactors, harvesting and centrifugation, cell lysis, and tangential flow filtration.

2-day training Hands-on lab training Toronto, ON
Go to the BioHubNet Training Platform
To enroll
  1. 01 Apply for ENGAGE Training Credits on the BioHubNet Training Platform If you are not already enrolled in the BioHubNet ENGAGE program
  2. 02 Log in to the BioHubNet Training Platform, open the “Learning Pathways” tab, and request to enroll.
Travel & accommodation support Available for eligible participants travelling to BioZone. Request travel support →
Assessment & instructor support Instructor-led assessment of practical skills, plus knowledge checks for the on-demand components.
01 — Pre-Requisites

Required preparation before the two-day lab workshop

Approximately 8 hours. Course access is provided after acceptance.

Lectures

Preparatory Lectures

Establish shared technical context and readiness.

02 — Schedule

2-day training agenda

Day 1 — Bioreactors & Harvesting

  • Introduction and safety briefing
  • Small scale bioreactors: components, assembly, autoclave prep, and probe calibration
  • Pilot scale bioreactors: fermentation prep and in situ sterilization
  • Hands-on culture: sampling, cell density, and dissolved oxygen
  • Harvesting and centrifugation: batch vs continuous, disc-stack centrifuge
  • Cell lysis and microfluidizer operation

Day 2 — Filtration & BioZone Tour

  • Introduction to filtration in downstream operations
  • Tangential Flow Filtration: principles and system components
  • Monitoring parameters: flow rate, flux, and TMP
  • TFF concentration process demonstration
  • Guided tour of the BioZone labs
  • Discussion and reflection
03 — Learning Outcomes

What you will be able to do

Bioreactors

How Bioreactors Operate

Learn how bioreactors operate and apply that learning to correctly assemble and disassemble bioreactor systems.

Scale

Small vs Pilot Scale

Understand the differences between small scale and large (pilot) scale bioreactors.

Downstream

Downstream Operations

Learn about downstream processing operations across harvesting, lysis, and filtration.

Centrifugation

Industrial Centrifugation

Understand the operation of an industrial scale continuous disc-stack centrifuge.

Cell Lysis

Mechanical Cell Lysis

Learn about the mechanical cell lysis method using a microfluidizer.

Filtration

Filtration & TFF

Understand the difference between depth filtration and cross-flow filtration, and how a tangential flow filtration (TFF) system operates.